Influence of Compost Amendments on Soil and Human Gastrointestinal Bacterial Communities during a Single Gardening Season

TL;DR

This study explores how different compost types used in gardening affect the gut bacteria of gardeners over a single season.

Contribution

The study provides empirical evidence linking compost amendments in gardening to detectable changes in human gut microbiota.

Findings

P compost users had the lowest gut microbiota diversity at T2/T3.

Ruminococcus 1 was less abundant in P compost users compared to CM or DMP users at T2.

CM compost users had a higher proportion of stool bacteria sourced from compost rather than soil.

Abstract

To measure associations between gardening with different compost amendments and the human gut microbiota composition, gardeners (n = 25) were provided with one of three types of compost: chicken manure (CM), dairy manure and plant material (DMP), or plant-based (P). Stool samples were collected before gardening (T1), after compost amendment (T2), and at peak garden harvest (T3). Compost and soil samples were collected. DNA was extracted, 16S rRNA libraries were established, and libraries were sequenced by Illumina MiSeq. Sequences were processed using mothur, and data were analyzed in R software version 4.2.2. Fast expectation-maximization microbial source tracking analysis was used to determine stool bacteria sources. At T2/T3, the gut microbiotas of P participants had the lowest Shannon alpha diversity, which was also the trend at T1. In stool from T2, Ruminococcus 1 were less abundant in the microbiotas of those using P compost as compared to those using CM or DMP. At T2, Prevotella 9 had the highest abundance in the microbiotas of those using CM compost. In participants who used CM compost to amend their gardening plots, a larger proportion of the human stool bacteria were sourced from CM compared to soil. Soil exposure through gardening was associated with a small but detectable change in the gardeners’ gut microbiota composition. These results suggest that human interactions with soil through gardening could potentially impact health through alterations to the gut microbiota.

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