# The complete chloroplast genome sequence and phylogenetic analysis of Asplenium antiquum Makino 1929, an Endangered species in Korea

## TL;DR

This study sequenced the complete chloroplast genome of the endangered Korean fern Asplenium antiquum and analyzed its evolutionary relationships.

## Contribution

The paper provides the first complete chloroplast genome sequence for Asplenium antiquum and its phylogenetic placement within the Aspleniaceae family.

## Key findings

- The chloroplast genome of A. antiquum is 150,690 bp long with a typical quadripartite structure.
- 114 genes were identified, including 84 protein-coding, 26 tRNA, and 4 rRNA genes.
- Phylogenetic analysis shows A. antiquum is closely related to A. prolongatum and A. nidus.

## Abstract

Asplenium antiquum Makino 1929 is one of the Endangered endemic species on the Korean Peninsula. The complete chloroplast of A. antiquum is 150,690 bp in length with typical quadripartite structure comprised of large single-copy region of (83,166 bp), a small single copy region (21,932 bp), and two inverted repeat regions, each 22,796 bp in length. 114 genes were detected in the chloroplast genome of A. antiquum, comprising 84 protein-encoding genes, 26 tRNA genes, and 4 rRNA genes. The phylogenetic analysis revealed a monophyletic relationship, placing A. antiquum as a sister to voth A. Prolongatum and A. nidus, forming a subclade of Asplenium species within the Aspleniaceae family. The genomic data obtained from this study will serve as valuable information for the species’ genetic classification of Asplenium.