# Synthetic approach to iodosulfuron-methyl and metsulfuron-methyl metabolites and their application for water analysis

## TL;DR

This study synthesizes metabolites of two herbicides and uses them to analyze water samples from the Vistula river.

## Contribution

A new synthetic approach to produce herbicide metabolites for water analysis is presented.

## Key findings

- Ten metabolites were synthesized and characterized using IR, NMR, and elemental analysis.
- A validated HPLC-MS/MS method was developed for separation and quantification of the compounds.
- The method was successfully applied to analyze water samples from the Vistula river.

## Abstract

A synthetic approach to ten metabolites of iodosulfuron-methyl sodium and metsulfuron-methyl was performed and reported in this study. The compounds of interest were prepared by controlled hydrolytic degradation of active substances or by de novo synthesis from commercially available triazine precursor 10. Obtained compounds were characterized by IR, NMR, and elemental analysis techniques. Metabolites and active substances were utilized during the development of a separation and quantification method using reversed-phase high-performance liquid chromatography coupled with tandem mass spectrometry. The validated method was applied for the analysis of all studied compounds in the extracts from water samples collected from the Vistula river (Toruń, Poland).

Synthesis of iodosulfuron-methyl and metsulfuron-methyl metabolites and their application for water sample analysis from the Vistula river are reported.