# Ozonated Sunflower Oil (OSO) Alleviates Inflammatory Responses in Oxazolone-Induced Atopic Dermatitis (AD)-Like Mice and LPS-Treated RAW 264.7 Cells

## TL;DR

Ozonated sunflower oil reduces inflammation in a mouse model of atopic dermatitis and in macrophage cells, suggesting it could be a potential treatment for AD-related skin conditions.

## Contribution

This study demonstrates the anti-inflammatory effects of ozonated sunflower oil in atopic dermatitis models.

## Key findings

- OSO reduced LPS-induced IL-1β and NO levels in RAW 264.7 cells.
- OSO alleviated AD-like symptoms and immune responses in oxazolone-treated mice.
- OSO inhibited the IL-4/STAT3/MAPK and NF-κB pathways.

## Abstract

Ozone, a highly reactive oxidant molecule, is widely used as a complementary therapy for various skin diseases, including wound healing, pressure ulcers, diabetic foot, and infections. However, there is limited research on the effectiveness of ozone for atopic dermatitis (AD). Ozonated sunflower oil (OSO) is an active ingredient obtained from partially ozonated sunflower oil (SO). OSO markedly reduced the LPS-induced increase in IL-1β and nitric oxide (NO) levels in RAW 264.7 mouse macrophage cells. Oxazolone (OXZ) was applied to hairless mice to induce AD-like skin symptoms and immune response. OSO significantly alleviated the OXZ-induced increases in the number of infiltrating mast cells, epidermal thickness, AD symptoms, thymic stromal lymphopoietin (TSLP), and filaggrin, as well as the serum levels of NO, IgE, IL-1β, and TNF-α. Furthermore, OSO inhibited the IL-4/STAT3/MAPK pathway and the expression of NF-κB. Our results suggest that OSO treatment could relieve AD-mediated skin damage through its anti-inflammatory and antioxidant activities. Therefore, it can be used as a therapeutic agent against AD-related skin diseases.