# Dataset of seminal plasma proteome of Nellore bulls with high and low percentage of abnormalities sperm

## TL;DR

This study identifies 297 seminal plasma proteins in Nellore bulls with varying sperm abnormality rates, offering new insights into bull fertility and reproductive biology.

## Contribution

The first comparative proteomic dataset of seminal plasma proteins in young Nellore bulls categorized by sperm abnormality percentages.

## Key findings

- A total of 297 proteins were identified in seminal plasma using LC-MS/MS.
- The dataset provides a comparative profile of proteins associated with sperm abnormalities in Nellore bulls.
- The findings contribute to understanding the role of seminal plasma proteins in bull fertility.

## Abstract

Bovine seminal plasma proteins perform several functions related to sperm function. Changes in the expression pattern or abundance of seminal proteins are related to changes in the fertilizing capacity of bulls. Considering the role of seminal plasma proteins in sperm function and animal reproduction, we investigated changes in the protein abundance profile in response to sperm morphological changes using a proteomic approach.

In our present investigation, we employed liquid chromatography coupled with mass spectrometry to elucidate the proteomic composition of seminal plasma obtained from Nellore bulls exhibiting varying percentages of sperm abnormalities. Following semen collection, seminal plasma was promptly isolated from sperm, and proteins were subsequently precipitated, enzymatically digested using porcine trypsin, and subjected to analysis utilizing the Acquity nano UHPLC System in conjunction with a mass spectrometer. This dataset encompasses a total of 297 proteins, marking the inaugural instance in which a comparative profile of seminal plasma proteins in young Nellore bulls, categorized by their sperm abnormality percentages, has been delineated using LC-MS/MS. The comprehensive nature of this dataset contributes pivotal proteomic insights, representing a noteworthy advancement in our understanding of the reproductive biology of the Nellore breed.