# The PreQuine Platform: A novel diagnostic tool for measuring glucose-6-phosphate dehydrogenase (G6PD) activity and hemoglobin concentration

## TL;DR

The PreQuine Platform is a new diagnostic tool that measures G6PD activity and hemoglobin concentration from a small blood sample using a mobile app and portable device.

## Contribution

The novel dual-analyte biosensor enables simultaneous measurement of G6PD activity and hemoglobin in the same blood sample.

## Key findings

- The PreQuine Platform showed significant agreement with reference methods for G6PD activity measurements.
- The device requires only 10μl of whole blood and uses a reflectance-based meter for analysis.
- Field trials are planned in Bangladesh, Cambodia, and the USA to evaluate performance and user acceptance.

## Abstract

Quantitative diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency is essential for the safe administration of 8-aminoquinoline based radical cure for the treatment of Plasmodium vivax infections. Here, we present the PreQuine Platform (IVDS, USA), a quantitative biosensor that uses a dual-analyte assay for the simultaneous measurement of Hemoglobin (Hgb) levels and G6PD enzyme activity within the same sample. The platform relies on a downloadable mobile application. The device requires 10μl of whole blood and works with a reflectance-based meter. Comparing the G6PD measurement normalized by Hgb of 12 samples from the PreQuine Platform with reference measurements methods (spectrophotometry, Pointe Scientific, USA and hemoglobin meter, HemoCue, Sweden) showed a positive and significant agreement with a slope of 1.0091 and an intercept of -0.0379 under laboratory conditions. Next steps will be to conduct field trials in Bangladesh, Cambodia, and the USA to assess diagnostic performance, user friendliness and acceptance.