# POLB Regulates Proliferation and Apoptosis of Bovine Primary Myocytes

## TL;DR

This study investigates how the POLB gene affects the growth and death of bovine muscle cells, finding that overexpression of POLB promotes cell death.

## Contribution

The study identifies POLB as a muscle development-related gene and reveals its pro-apoptotic effect in bovine primary myocytes.

## Key findings

- Overexpression of POLB promotes apoptosis in bovine primary myocytes.
- POLB overexpression affects the expression of the CASP9 gene involved in apoptosis.

## Abstract

This study focused on the DNA polymerase β (POLB) gene, which belongs to the DNA polymerase X family involved in DNA replication, repair, recombination, and cell cycle regulation. POLB was identified as a muscle development-related gene through gene screening and gene expression analysis methods. Further validation in bovine primary myocytes showed that overexpression of POLB promoted apoptosis, while gene knockdown had no significant effect. Analysis of related genes revealed that POLB overexpression affected the expression of the CASP9 gene, which is involved in the apoptotic pathway.

DNA polymerase β (DNA polymerase beta (POLB)) belongs to a member of the DNA polymerase X family, mainly involved in various biological metabolic processes, such as eukaryotic DNA replication, DNA damage repair, gene recombination, and cell cycle regulation. In this study, the muscle development-related gene POLB was screened by selection signature and RNA-seq analysis and then validated for the proliferation and apoptosis of bovine primary myocytes. It was also found that overexpression of the POLB gene had a pro-apoptosis effect, but interfering with the expression of the gene had no significant effect on cells. Then, the analysis of related apoptotic genes revealed that POLB overexpression affected CASP9 gene expression.