# Apoptosis in Postmortal Tissues of Goat Spinal Cords and Survival of Resident Neural Progenitors

## TL;DR

This study shows that neural progenitor cells in goat spinal cords remain viable for up to 54 hours after death, making them a potential source for tissue repair.

## Contribution

The study provides new evidence that postmortal spinal cords can be a source of viable neural progenitor cells for therapeutic use.

## Key findings

- TUNEL-positive cells did not increase in areas with GD2-positive neural progenitors over 54 hours postmortem.
- Active caspase 3 levels increased moderately over time, but apoptosis was rare among CD24/GD2-positive cells.
- Postmortal spinal cords contain viable allogenic neural progenitor cells suitable for harvesting.

## Abstract

Growing demand for therapeutic tissue repair recurrently focusses scientists’ attention on critical assessment of postmortal collection of live cells, especially stem cells. Our study aimed to assess the survival of neuronal progenitors in postmortal spinal cord and their differentiation potential. Postmortal samples of spinal cords were obtained from human-sized animals (goats) at 6, 12, 24, 36, and 54 h after slaughter. Samples were studied by immunohistology, differentiation assay, Western blot and flow cytometry for the presence and location of GD2-positive neural progenitors and their susceptibility to cell death. TUNEL staining of the goat spinal cord samples over 6–54 h postmortem revealed no difference in the number of positive cells per cross-section. Many TUNEL-positive cells were located in the gray commissure around the central canal of the spinal cord; no increase in TUNEL-positive cells was recorded in either posterior or anterior horns of the gray matter where many GD2-positive neural progenitors can be found. The active caspase 3 amount as measured by Western blot at the same intervals was moderately increasing over time. Neuronal cells were enriched by magnetic separation with antibodies against CD24; among them, the GD2-positive neural progenitor subpopulation did not overlap with apoptotic cells having high pan-caspase activity. Apoptotic cell death events are relatively rare in postmortal spinal cords and are not increased in areas of the neural progenitor cell’s location, within measured postmortal intervals, or among the CD24/GD2-positive cells. Data from our study suggest postmortal spinal cords as a valuable source for harvesting highly viable allogenic neural progenitor cells.