# Acoustofluidic scanning fluorescence nanoscopy with a large field of view

## TL;DR

This paper introduces a new imaging technique that achieves high-resolution fluorescence imaging over a large area using acoustofluidic control.

## Contribution

The novel acoustofluidic scanning fluorescence nanoscope enables large-field, high-resolution imaging without compromising signal strength.

## Key findings

- The nanoscope achieves ~5 times fluorescent signal enhancement without altering the field of view.
- It resolves structures beyond the capabilities of conventional fluorescence microscopes.
- The method is suitable for diverse applications in medicine and biomedical research.

## Abstract

Large-field nanoscale fluorescence imaging is invaluable for many applications, such as imaging subcellular structures, visualizing protein interactions, and high-resolution tissue imaging. Unfortunately, conventional fluorescence microscopy requires a trade-off between resolution and field of view due to the nature of the optics used to form the image. To overcome this barrier, we developed an acoustofluidic scanning fluorescence nanoscope that simultaneously achieves superior resolution, a large field of view, and strong fluorescent signals. The acoustofluidic scanning fluorescence nanoscope utilizes the superresolution capabilities of microspheres that are controlled by a programmable acoustofluidic device for rapid fluorescence enhancement and imaging. The acoustofluidic scanning fluorescence nanoscope resolves structures that cannot be resolved with conventional fluorescence microscopes with the same objective lens and enhances the fluorescent signal by a factor of ~5 without altering the field of view of the image. The improved resolution realized with enhanced fluorescent signals and the large field of view achieved via acoustofluidic scanning fluorescence nanoscopy provides a powerful tool for versatile nanoscale fluorescence imaging for researchers in the fields of medicine, biology, biophysics, and biomedical engineering.