# Formulation of a Novel Polymeric Hydrogel Membrane for Periodontal Tissue Regeneration Using Tricalcium Phosphate-Alginate Reinforcement

## TL;DR

This study creates a new hydrogel membrane reinforced with tricalcium phosphate and alginate for periodontal tissue regeneration, showing it is safe and supports cell attachment.

## Contribution

A novel TCP-alginate reinforced hydrogel membrane is developed for periodontal tissue regeneration with demonstrated hemocompatibility.

## Key findings

- SEM analysis showed a structure favorable for cell attachment in the TCP-alginate membrane.
- FTIR confirmed the presence of β-TCP phases in the membrane.
- The membrane had a hemolysis rate under 5%, indicating good hemocompatibility.

## Abstract

Background: The primary goal of periodontal therapy is to facilitate the regeneration of tissues damaged by periodontal disease. In recent years, there has been a growing utilization of guided tissue regeneration (GTR) membranes with bioabsorbable properties as these membranes are increasingly employed to guide the growth of gingival tissue away from the root surface. Both resorbable and non-resorbable membranes currently employed act as physical barriers, preventing the ingrowth of connective and epithelial tissues into the defect and thereby facilitating periodontal tissue regeneration.

Objective: This study aimed to develop a polymeric hydrogel membrane reinforced with tricalcium phosphate (TCP)-alginate and assess its potential for periodontal regeneration.

Materials and methods: TCP nanoparticles were incorporated into the alginate mixture to form TCP alginate. Subsequently, the mixture was cross-linked with calcium chloride to produce a TCP-alginate polymeric hydrogel membrane. The membrane underwent hemocompatibility analysis, and also scanning electron microscopy and Fourier-transform infrared (FTIR) spectroscopy analyses were done.

Results: The SEM analysis revealed granulations and a bonded thread-like structure in the membrane, indicative of favorable conditions for cell attachment necessary for periodontal regeneration. FTIR analysis showed characteristic peaks in the spectrum, including those attributed to phosphate ion (PO4-3) at 1000.85 cm-1 and 600 cm-1, indicating the presence of β-TCP phases. Hemocompatibility assessment demonstrated a hemolysis rate of less than 5% for the TCP-alginate membrane, which is found to be within the limits.

Conclusion: The developed TCP-reinforced alginate membrane exhibited hemocompatibility and safety, suggesting its suitability for utilization in periodontal therapy as an effective regenerative material.