# Downregulated PDIA3P1 lncRNA Impairs Trophoblast Phenotype by Regulating Snail and SFRP1 in PE

## TL;DR

A pseudogene called PDIA3P1 is reduced in preeclampsia and affects placental cell function by regulating key proteins, offering new insights into the condition's causes.

## Contribution

This study identifies PDIA3P1 as a novel pseudogene involved in preeclampsia by modulating trophoblast behavior through HuR and Snail.

## Key findings

- PDIA3P1 is significantly downregulated in preeclamptic placental tissues.
- PDIA3P1 suppression reduces trophoblast proliferation, invasion, and migration.
- PDIA3P1 stabilizes Snail expression via binding to HuR, affecting placental function in preeclampsia.

## Abstract

Preeclampsia (PE) manifests as a pregnancy-specific complication arising from compromised placentation characterized by inadequate trophoblast invasion. A growing body of evidence underscores the pivotal involvement of pseudogenes, a subset of long noncoding RNAs, in the pathological processes of PE. This study presents a novel finding, demonstrating a significant downregulation of the pseudogene PDIA3P1 in PE placental tissues compared to normal tissues. In vitro functional assays revealed that suppressing PDIA3P1 hindered trophoblast proliferation, invasion, and migration, concurrently upregulating the expression of secreted frizzled-related protein 1 (SFRP1). Further exploration of the regulatory role of PDIA3P1 in PE, utilizing human trophoblasts, established that PDIA3P1 exerts its function by binding to HuR, thereby enhancing the stability of Snail expression in trophoblasts. Overall, our findings suggest a crucial role for PDIA3P1 in regulating trophoblast properties and contributing to the pathogenesis of PE, offering potential targets for prognosis and therapeutic intervention.