# Viral DNA in submandibular gland tissue with an inflammatory disorder

## TL;DR

This study investigates whether viruses trigger IgG4 overexpression in chronic sialadenitis of the submandibular gland.

## Contribution

The study provides insights into the prevalence of DNA viruses in submandibular gland tissues with and without IgG4 overexpression.

## Key findings

- Seven viruses were detected in 48 out of 61 submandibular gland tissue samples.
- EBV DNA was more common in IgG4-positive samples, while B19V was more common in IgG4-negative samples.
- None of the studied viruses were found to trigger IgG4 overexpression in chronic sialadenitis.

## Abstract

The etiology behind different types of chronic sialadenitis (CS), some of which exhibit IgG4 overexpression, is unknown. Further, IgG4-related disease (IgG4-RD) commonly affects the submandibular gland, but its relationship to IgG4-overexpressing CS, and the antigen triggering IgG4 overexpression, remain unknown.

By qPCR, we assessed the presence of 21 DNA-viruses causing IgG4 overexpression in submandibular gland tissue from patients with IgG4-positive and IgG4-negative CS. Healthy submandibular glands and glands with sialolithiasis without CS were used as controls. We examined the distribution of HHV-7, HHV-6B and B19V DNA, within virus PCR-positive tissues with RNAscope in-situ hybridization (RISH).

We detected DNA from seven viruses in 48/61 samples. EBV DNA was more prevalent within the IgG4-positive samples (6/29; 21%) than the IgG4-negative ones (1/19; 5.3%). B19V DNA was more prevalent within the IgG4-negative samples (5/19; 26%) than the IgG4-positive ones (4/29; 14%). The differences in virus prevalence were not statistically significant. Of the IgG4-RD samples (n = 3) one contained HHV-6B DNA. RISH only showed signals of HHV-7.

None of the studied viruses are implicated as triggering IgG4-overexpression in CS. Although our results do not confirm viral etiology in the examined conditions, they provide valuable information on the prevalence of viruses in both diseased and healthy submandibular gland tissue.