# Protective Efficacy of Lyophilized Vesicular Stomatitis Virus–Based Vaccines in Animal Model

## TL;DR

This study tested how well freeze-dried vaccines based on vesicular stomatitis virus protect guinea pigs and found that vaccine stability can be improved through lyophilization.

## Contribution

The study demonstrates protective immunity of lyophilized/reconstituted vesicular stomatitis virus-based vaccines in an animal model.

## Key findings

- Lyophilization increased the stability of the vaccines.
- Protective immunity was demonstrated in guinea pigs after reconstitution of the lyophilized vaccines.

## Abstract

We evaluated the in vitro effects of lyophilization for 2 vesicular stomatitis virus–based vaccines by using 3 stabilizing formulations and demonstrated protective immunity of lyophilized/reconstituted vaccine in guinea pigs. Lyophilization increased stability of the vaccines, but specific vesicular stomatitis virus–based vaccines will each require extensive analysis to optimize stabilizing formulations.