Correction: The rapid-tome, a 3D-printed microtome, and an updated hand-sectioning method for high-quality plant sectioning
D. J. Thomas, J. Rainbow, L. E. Bartley
Abstract
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
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Taxonomy
TopicsAlgorithms and Data Compression · DNA and Biological Computing
Correction: Plant Methods (2023) 19:12 10.1186/s13007-023-00986-3
The author of the original article [1] would like to include two supplementary files as mentioned below.
- A new 3D print.stl file, “Rapid-Tome Sled Thick Washer Compatible_New.stl”. This file is for the sliding piece that holds the blade for the RapidTome. It makes two small adjustments to the previous “Rapid-Tome Sled.stl” file.
- The first is to slightly reduce the size of the lower part of the sled so that the sled does not make contact with the handle of the Rapid-Tome during the sectioning motion.
- The second is to make the part of the sled that holds the blade slightly thinner. This change accommodates a thicker washer that surrounds the sample opening and upon which the blade slides, as the generally available washers (such as through Amazon) seem to be a few millimeters thicker than the one which we designed the Rapid-Tome for.
- A video that demonstrates the assembly of the Rapid-Tome after printing.
Notes: In addition, our interactions with readers and our own experience lead us to point out two of the non-printed components that seem to be particularly important for users to assemble and use their own device.
- Please note that PTFE coated blades, frequently changed out, seem to be especially important for thin sections.
- Please pay particular attention to the firmness of the foam tape piece used to hold the blade. Low-density, easily compressed foam will NOT hold the blade firmly enough. Please use high density, or “closed cell” PVC “foam” or “sponge” tape. Tape dimensions should be ¼ in. thick X 1/2 in. wide (or 6.4 mm X 13 cm). You will cut a piece that fits along the bottom of the “clamp.”
Supplementary Information
Additional file 1. Rapid-Tome Sled.stl.Additional file 2. RapidTome assembly video.
The reference list from the paper itself. Each links out to its DOI / PubMed record.