# Improving the efficiency of estrus synchronization in cows

## TL;DR

This study found that using electrical stimulation and certain substances during estrus synchronization improves cow fertility in Kazakhstan.

## Contribution

The novel finding is that electrical stimulation combined with specific biologically active substances enhances estrus synchronization effectiveness in cows.

## Key findings

- Cows in group 6 had significantly higher E2 levels on day 10 compared to other groups.
- Fertility rates were significantly higher in group 6 than in groups 2, 3, 4, and the control.
- Electrical stimulation and biological substances positively affect reproductive performance in beef cattle.

## Abstract

This study aimed to determine the effects of biologically active substances and electrical stimulation of the uterus in cows on the effectiveness of estrus synchronization.

Ninety (n = 90) Kazakh white-headed cows were synchronized with two injections of gonadotropin-releasing hormone on days 0 and 9 and prostaglandin F2α on day 7. The cows were divided into six groups and, during the protocol, treated with biologically active substances (Tetramag, Selevetum, antiseptic-stimulator Dorogov 2 fraction, groups 2, 3, and 4). Cows in groups 5 and 6 were treated with the same substances but additionally had electrical stimulation of the uterus, while cows in group 1 were left untreated and served as a control.

The results have shown that on Day 0, no differences were observed in E2 concentrations between the groups. However, on the 10th day, a significant disparity was noted in the E2 level among cows in group 6 compared to groups 2, 3, 4, and the control group. Conversely, no significant differences were observed between groups 5 and 6. Likewise, the fertility rate in cows from group 6 was significantly higher compared to groups 2, 3, 4, and the control group, with no significant differences between groups 5 and 6.

It can be concluded that the utilization of electrical stimulation of the uterus and the inclusion of certain biological substances during the estrus synchronization protocol demonstrate a positive effect on the reproductive performance of beef cattle in Kazakhstan.