# The Dual-Targeted Fusion Inhibitor Clofazimine Binds to the S2 Segment of the SARS-CoV-2 Spike Protein

## TL;DR

This paper shows that Clofazimine, a drug repurposed for treating COVID-19, binds to a key part of the SARS-CoV-2 virus's spike protein, potentially inhibiting viral fusion.

## Contribution

The study identifies Clofazimine's specific binding site on the S2 segment of the SARS-CoV-2 Spike protein and supports its broad-spectrum antiviral potential.

## Key findings

- Clofazimine binds reliably to the S2 segment of the SARS-CoV-2 Spike protein.
- Molecular docking reveals two potential binding sites for fusion inhibitors on the S2 segment.
- The proposed binding site aligns with structures of other coronaviruses, explaining Clofazimine's broad antiviral activity.

## Abstract

Clofazimine and Arbidol have both been reported to be effective in vitro SARS-CoV-2 fusion inhibitors. Both are promising drugs that have been repurposed for the treatment of COVID-19 and have been used in several previous and ongoing clinical trials. Small-molecule bindings to expressed constructs of the trimeric S2 segment of Spike and the full-length SARS-CoV-2 Spike protein were measured using a Surface Plasmon Resonance (SPR) binding assay. We demonstrate that Clofazimine, Toremifene, Arbidol and its derivatives bind to the S2 segment of the Spike protein. Clofazimine provided the most reliable and highest-quality SPR data for binding with S2 over the conditions explored. A molecular docking approach was used to identify the most favorable binding sites on the S2 segment in the prefusion conformation, highlighting two possible small-molecule binding sites for fusion inhibitors. Results related to molecular docking and modeling of the structure–activity relationship (SAR) of a newly reported series of Clofazimine derivatives support the proposed Clofazimine binding site on the S2 segment. When the proposed Clofazimine binding site is superimposed with other experimentally determined coronavirus structures in structure–sequence alignments, the changes in sequence and structure may rationalize the broad-spectrum antiviral activity of Clofazimine in closely related coronaviruses such as SARS-CoV, MERS, hCoV-229E, and hCoV-OC43.