# Screening and Preliminary Identification of Asparagus officinalis Varieties under Low-Temperature Stress

## TL;DR

This study identifies a gene that may help asparagus respond to cold, improving its cultivation in spring.

## Contribution

The study identifies AoMYB56 as a potential regulator of ABA in asparagus under low-temperature stress.

## Key findings

- AoMYB56 gene was identified as a candidate involved in low-temperature response in asparagus.
- Transcriptome sequencing and hormone analysis revealed ABA's importance in cold response.
- Phylogenetic and subcellular analyses supported AoMYB56's potential regulatory role.

## Abstract

To meet the large demand for Asparagus officinalis in the spring market and improve the economic benefits of cultivating asparagus, we explored the molecular mechanism underlying the response of A. officinalis to low temperature. First, “Fengdao No. 1” was screened out under low-temperature treatment. Then, the transcriptome sequencing and hormone detection of “Fengdao No. 1” and “Grande” (control) were performed. Transcriptome sequencing resulted in screening out key candidate genes, while hormone analysis indicated that ABA was important for the response to low temperature. The combined analysis indicated that the AoMYB56 gene may regulate ABA in A. officinalis under low temperature. And the phylogenetic tree was constructed, and subcellular localisation was performed. From these results, we speculated that the AoMYB56 gene may regulate ABA in A. officinalis. The results of this research provide a theoretical basis for the further exploration of low-temperature response in A. officinalis.