# B cell immunofocusing and repriming in two HIV-1 Env immunization regimens

## TL;DR

This study compares two HIV vaccine strategies and how they affect B-cell responses in non-human primates.

## Contribution

The study reveals how different HIV-1 Env immunization regimens influence B-cell immunofocusing and repriming.

## Key findings

- A multiclade, multivalent regimen led to B-cell responses converging on shared epitopes.
- A sequential regimen with non-stabilized and stabilized gp140 trimers reprimed B-cell responses.
- Changes in immunogen format significantly impact B-cell memory responses.

## Abstract

Diverse and rapidly mutating viruses pose challenges to immunogen and vaccine design. In this study, we evaluated the ability of memory B-cells obtained from two independent NHP trials to cross-react with individual HIV-1 vaccine components of two different multivalent immunization strategies. We demonstrated that while an HIV-1 Env multiclade, multivalent immunization regimen resulted in a dominant memory B-cell response that converged toward shared epitopes, in a sequential immunization with clonally-related non-stabilized gp140 HIV-1 Envs followed by SOSIP-stabilized gp140 trimers, the change in immunogen format resulted in repriming of the B-cell response.