# Rapid and quantitative detection of DNA hybridization using a simplified Fabry–Perot interferometric biosensor

## TL;DR

A new fiber-optic sensor quickly and accurately detects DNA hybridization, even with mismatched sequences, offering a fast and reusable tool for DNA analysis.

## Contribution

A simplified, cost-effective Fabry–Perot biosensor for rapid, quantitative DNA hybridization detection with high mismatch discrimination.

## Key findings

- The sensor distinguishes DNA sequences with 2 and 7 mismatches effectively.
- It achieves results within 6 minutes and shows high repeatability and reusability.
- Performance was validated through multiple hybridization and regeneration cycles.

## Abstract

This study introduces a miniaturized fiber-optic Fabry–Perot (FP) interferometric biosensor, distinctively engineered for cost-effective, rapid, and quantitative DNA sequence detection. By leveraging the interference patterns generated within a Fabry–Perot microcavity, our sensor precisely monitors DNA hybridization events in real-time. We have verified the sensor's biofunctionalization via fluorescent labeling and have extensively validated its performance through numerous hybridization and regeneration cycles with 1 μM single-stranded DNA (ssDNA) solutions. Demonstrating remarkable repeatability and reusability, the sensor effectively discerns ssDNA sequences exhibiting varying degrees of mismatches. Its ability to accurately distinguish between sequences with 2 and 7 mismatches underscores its potential as a valuable asset for swift DNA analysis. Characterized by its rapid response time—typically yielding results within 6 minutes—and its adeptness at mismatch identification, our biosensor stands as a potent tool for facilitating accelerated DNA diagnostics and research.

The simple-structured Fabry–Perot interferometric biosensor is capable of rapid and quantitative detection of DNA hybridization.