The identification of high-performing antibodies for RNA-binding protein TIA1 for use in Western Blot, immunoprecipitation and immunofluorescence

TL;DR

This study identifies reliable antibodies for the RNA-binding protein TIA1, which is linked to neurodegenerative diseases like ALS and FTD.

Contribution

The study provides a validated resource of high-performing anti-TIA1 antibodies for common experimental techniques.

Findings

Twelve commercial anti-TIA1 antibodies were tested for performance in Western Blot, immunoprecipitation, and immunofluorescence.

Several high-performing antibodies were identified using knockout and isogenic cell lines for comparison.

The results offer a practical guide for selecting suitable antibodies for TIA1 research.

Abstract

A member of the RNA-binding protein family, T-cell intracellular antigen-1 (TIA1) regulates mRNA translation and splicing as well as cellular stress by promoting stress granule formation. Variants of the
TIA1 gene have implications in neurogenerative disorders including amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Reproducible research on TIA1 would be enhanced with the availability of high-quality anti-TIA1 antibodies. In this study, we characterized twelve TIA1 commercial antibodies for Western Blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. We identified many high-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs.

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