# Influence of ethnic origin on the clinical characteristics and intestinal flora of irritable bowel syndrome: a prospective study between Han and Tibetan patients

## TL;DR

This study compares the symptoms and gut bacteria of Han and Tibetan people with IBS, finding that Tibetans experience more bloating and have different gut microbes.

## Contribution

The study reveals ethnic differences in IBS symptoms and gut microbiota, particularly highlighting bloating and Blautia levels in Tibetan patients.

## Key findings

- Tibetan patients with IBS experience more bloating than Han patients.
- The Firmicutes/Bacteroidetes ratio is significantly lower in Tibetan patients.
- Blautia levels are reduced in Tibetan patients and negatively correlated with bloating scores.

## Abstract

Few studies have focused on the clinical characteristics and intestinal flora of Tibetan patients with irritable bowel syndrome (IBS). The study aimed to compare the difference of between Tibetan and Han patients with IBS.

Patients who met inclusion and exclusion criteria were divided into the Tibet and Han groups. A simplified Gastrointestinal Symptom Rating Scale (GSRS)-based questionnaire was used to assess the IBS severity. Fecal samples from all subjects were collected for the analysis of gut microbiota using 16sRNA Illumina sequencing.

No significant difference was found in the total symptom scores between two groups. However, Tibetans with IBS are more prone to bloating than Hans (17.41% vs 9.09%, p < 0.001). A profit shift in the gut microbiota was shown between the two groups. The ratio of Firmicutes/Bacteroidetes was significantly lower in the Tibet group than in the Han group (2.954 ± 0.78 vs 8.23 ± 2.04, p = 0.004). In the Tibet group, the level of the genus Blautia decreased significantly compared to the Han group, and there was a significant negative correlation between the level of Blautia and the bloating scores (Pearson r = −0.33, p = 0.025).

The characteristics of Tibetan patients differ from those of Han patients with IBS, not only in terms of the clinical symptoms, but also in the characteristics of intestinal flora. Tibetans with IBS are more prone to bloating, which might be due to the different gut microbiota. The genus Blautia may play a role in this mechanism.