# Generation and characterization of temperature-sensitive alleles encoding GPI anchored proteins Psu1 and Dfg502 in Schizosaccharomyces pombe

## TL;DR

This paper studies yeast proteins involved in cell wall formation and finds their roles in cell separation and viability.

## Contribution

The study introduces new temperature-sensitive alleles for analyzing GPI-anchored proteins in S. pombe.

## Key findings

- Psu1 is essential for cell separation, proper cell shape, and viability.
- Dfg501 and Dfg502 have redundant and essential roles in cell separation and viability.

## Abstract

Glycosyl-phosphatidylinositol (GPI) anchored proteins are implicated in remodeling of the yeast cell wall during growth and division.
Schizosaccharomyces pombe
proteins,
Psu1
,
Dfg501
, and
Dfg502
are predicted GPI anchored proteins with likely cell wall modifying activity. Here, we isolated and characterized null and temperature-sensitive alleles that will allow further analysis of the function of these proteins and
S. pombe
cell wall formation. Our data confirm that
Psu1
is necessary for cell separation, maintaining proper cell shape, and viability. Additionally, we found that
Dfg501
and
Dfg502
share a redundant and essential function necessary for cell separation and viability.