Enhanced resistance to heat and fungal infection in transgenic Trichoderma via over-expressing the HSP70 gene
Yanhua Huang, Changfa Liu, Xuexue Huo, Xianzhi Lai, Wentao Zhu, Yongren Hao, Zehui Zheng, Kai Guo
TL;DR
This study shows that overexpressing the HSP70 gene in Trichoderma improves its resistance to heat and fungal infections.
Contribution
The study identifies and experimentally validates the role of HSP70 in enhancing Trichoderma's stress resistance through transcriptomic and functional analyses.
Findings
Overexpression of HSP70 in Trichoderma enhances growth, antioxidant capacity, and antifungal activity.
HSP70 activates genes involved in antibiotic synthesis and stress response pathways.
Transcriptomic analysis reveals HSP70's role in regulating secondary metabolite biosynthesis under heat stress.
Abstract
Heat stress is one of the major abiotic stresses affecting the growth, sporulation, colonization and survival of Trichoderma viride. This study aimed to gain a better insight into the underlying mechanism governing the heat stress response of T. viride Tv-1511. We analysed the transcriptomic changes of Tv-1511 under normal and heat stress conditions using RNA sequencing. We observed that Tv-1511 regulates the biosynthesis of secondary metabolites through a complex network of signalling pathways. Additionally, it significantly activates the anti-oxidant defence system, heat shock proteins and stress-response-related transcription factors in response to heat stress. TvHSP70 was identified as a key gene, and transgenic Tv-1511 overexpressing TvHSP70 (TvHSP70-OE) was generated. We conducted an integrated morphological, physiological and molecular analyses of the TvHSP70-OE and wild-type…
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Taxonomy
TopicsEducation and Islamic Studies · Early Childhood Education and Development