# The chromosome-level genome assembly of the giant dobsonfly Acanthacorydalis orientalis (McLachlan, 1899)

## TL;DR

Researchers created a high-quality genome assembly for the giant dobsonfly, which is important for water quality monitoring and conservation.

## Contribution

The study provides a chromosome-level genome assembly for Acanthacorydalis orientalis using Oxford Nanopore and Hi-C technologies.

## Key findings

- The genome assembly has a size of 547.98 Mb with high contiguity and completeness (99.01%).
- 10,977 protein-coding genes were identified, with 84% functionally annotated.
- The genome contains 44.86% repeat sequences and is anchored onto 13 pseudo-chromosomes.

## Abstract

Acanthacorydalis orientalis (McLachlan, 1899) (Megaloptera: Corydalidae) is an important freshwater-benthic invertebrate species that serves as an indicator for water-quality biomonitoring and is valuable for conservation from East Asia. Here, a high-quality reference genome for A. orientalis was constructed using Oxford Nanopore sequencing and High throughput Chromosome Conformation Capture (Hi-C) technology. The final genome size is 547.98 Mb, with the N50 values of contig and scaffold being 7.77 Mb and 50.53 Mb, respectively. The longest contig and scaffold are 20.57 Mb and 62.26 Mb in length, respectively. There are 99.75% contigs anchored onto 13 pseudo-chromosomes. Benchmarking Universal Single-Copy Orthologs (BUSCO) analysis showed that the completeness of the genome assembly is 99.01%. There are 10,977 protein-coding genes identified, of which 84.00% are functionally annotated. The genome contains 44.86% repeat sequences. This high-quality genome provides substantial data for future studies on population genetics, aquatic adaptation, and evolution of Megaloptera and other related insect groups.