# Synthesis of Zinc Oxide Nanoparticles From Cymodocea Serrulata Leaf Extract and Their Biological Activities

## TL;DR

This paper describes a green method to synthesize zinc oxide nanoparticles using Cymodocea serrulata leaf extract and tests their antibacterial properties.

## Contribution

The study introduces a novel, eco-friendly synthesis method for zinc oxide nanoparticles using a marine plant extract.

## Key findings

- Zinc oxide nanoparticles synthesized using Cymodocea serrulata leaf extract have an average size below 100 nm.
- The UV spectrum analysis showed a notable absorbance peak at 47.0 nm for the synthesized nanoparticles.
- The nanoparticles exhibited antibacterial properties in in vitro tests.

## Abstract

Introduction

The utilization of Cymodocea serrulata for the eco-friendly synthesis of zinc oxide nanoparticles, which contain distinguishable nanostructures, presents a cost-effective and environmentally sustainable alternative for producing zinc nanoparticles. The production process of zinc nanoparticles are rich in phytochemicals, which can serve as stabilizing and reducing agents. Zinc nanoparticles can easily pass through bacterial cell walls and reach all cellular components. C. serrulata, is a small submerged angiosperm commonly found in submerged and tidal coastal environments.

Aim

Analysis of the biological activities of zinc oxide nanoparticles made from C. serrulata leaf extract.

Materials and Methods

Dry leaves of C. serrulata were ground into a powder, which was then placed into a conical flask and filled with water. Subsequently, the color of the mixture turned black. Next, a 20 mm piece of ZnO was dissolved in a 60 ml sample of distilled water to prepare the metal solution. Following this, a wavelength scan ranging from 200 to 700 nm was conducted using ultraviolet (UV) spectroscopy. After shaking the solution for an hour, a final reading was taken across the UV spectrum. The synthetic sample should also be centrifuged to remove any pellets and subsequently dried in a hot air oven.

Result

Using nanoscale profiling, the average particle size was measured and found to be less than 100 nm, specifically UV spectrum analysis revealed a notable absorbance value of 47.0 nm, at different angles within the peak height. The wavelength range of the zinc nanoparticles was observed to be between 250 and 350 nm.

Conclusion

The antibacterial properties of ZnO NPs have been demonstrated through in vitro investigations, indicating their potential application in in vivo studies.