# One Step Forwards in Knowledge of Blossom Blight Brown Rot Disease: Monilinia spp. SSR Marker Database

## TL;DR

This paper introduces a free database of microsatellite markers for three fungal pathogens causing brown rot blossom blight in Europe.

## Contribution

The novelty is the creation of a centralized, freely accessible SSR marker database for Monilinia spp. pathogens.

## Key findings

- The database contains 39,216 SSR motifs and 26,366 markers for M. fructigena, M. laxa, and M. fructicola.
- Eight in silico-designed primers were experimentally validated and included in the database.
- The database includes detailed information such as primer sequences, fragment sizes, and genome coordinates.

## Abstract

A freely available Monilinia spp. marker database was created, containing microsatellite (SSR) data of the three most essential European fungal pathogens: M. fructigena, M. laxa, and M. fructicola. These pathogens cause brown rot blossom blight. Microsatellites were identified using the bioinformatics tool Genome-wide Microsatellite Analyzing Toward Application (GMATA). The database provides information about SSR markers: forward and reverse sequences of the primers, fragment sizes, SSR motifs (and repeats), and the exact locations with the coordinates in the reference genome. This database currently contains information about 39,216 SSR motifs and 26,366 markers. In total, eight primers generated in silico were validated experimentally and they are marked in the database. All scientists can join this collaboration by adding their experimental data. This database is the initial start of organizing Monilinia spp. molecular data worldwide and, in the future, it could be extended by adding more molecular and genomic information.