# Characterizing a standardized BioPart for BAG-specific expression in C. elegans

## TL;DR

This paper introduces a standardized DNA part for specific gene expression in C. elegans, useful for biological engineering.

## Contribution

A short, standardized promoter for BAG-specific expression in C. elegans is characterized and made accessible for transgene design.

## Key findings

- Pflp-17 enables bright BAG-specific expression in both hermaphrodite and male C. elegans.
- Cloning vectors for gfp and mScarlet expression using Pflp-17 are developed and compatible with various transgene methods.
- The promoter is incorporated into an online transgene design tool for easy use.

## Abstract

Biological parts (BioParts) are modular and standardized DNA sequences that encode biological functions and contribute to the efficient biological engineering of complex systems. Here, we characterize a short BioPart (P

flp-17

, 300 bp) for bright multicopy and single-copy BAG-specific expression starting from the gastrula stage in hermaphrodite and male
C. elegans
. We have generated standardized P

flp-17

cloning vectors for BAG-specific
gfp
and
mScarlet
expression compatible with extra-chromosomal arrays and for single-copy transgene insertion. The short P

flp-17

promoter is easy to generate by gene synthesis and has been incorporated into our online transgene design tool (www.wormbuilder.org/transgenebuilder
)
.