# The complete mitogenome of the entomopathogenic fungus Metarhizium pinghaense 15R

## TL;DR

This paper reports the complete mitochondrial genome of the fungus Metarhizium pinghaense 15R, which is effective against aphids and could aid in biological research.

## Contribution

The study provides the first complete mitogenome assembly and annotation for Metarhizium pinghaense 15R, including gene structure and phylogenetic insights.

## Key findings

- The mitogenome includes genes for ATP synthase, cytochrome oxidase, and NADH dehydrogenase, along with rRNA and tRNA genes.
- Five genes contain introns that may encode endonucleases and ribosomal protein S3.
- Phylogenetic analysis places M. pinghaense 15R within the Clavicipitaceae family, closely related to other Metarhizium species.

## Abstract

In this study, the complete mitogenome of the entomopathogenic fungus Metarhizium pinghaense 15 R, which is highly virulent to aphids and was isolated from Korean soil, was assembled and annotated for three ATP synthase subunits (atp6, atp8, and atp9), three cytochrome oxidase subunits (cox1, cox2, and cox3), apocytochrome b (cob), seven subunits of NADH dehydrogenase (nad1, nad2, nad3, nad4, nad4L, nad5, and nad6), two ribosomal RNAs (rnl and rns), and 19 tRNA genes. Five genes were carrying a total of eight introns, and they may encode ribosomal protein S3, LAGLIDADG and GIY-YIG endonucleases. Phylogenetic analysis based on the mitochondrial nucleotide sequence confirmed that the M. pinghaense 15 R is a member of the Clavicipitaceae, and is closely related to the species M. anisopliae, M. robertsii, and M. brunneum. The mtDNA base sequence of the M. pinghaense 15 R strain reported in this study is thought to be useful for biological resource genetic data.