Shotgun metagenomics and systemic targeted metabolomics highlight indole-3-propionic acid as a protective gut microbial metabolite against influenza infection
Séverine Heumel, Vinícius de Rezende Rodovalho, Charlotte Urien, Florian Specque, Patrícia Brito Rodrigues, Cyril Robil, Lou Delval, Valentin Sencio, Amandine Descat, Lucie Deruyter, Stéphanie Ferreira, Marina Gomes Machado, Adeline Barthelemy, Fabiola Silva Angulo, Joel. T Haas
TL;DR
This study shows that the gut microbiome produces a compound called indole-3-propionic acid, which helps protect against influenza by reducing viral load and inflammation.
Contribution
The study identifies indole-3-propionic acid as a protective gut microbial metabolite against influenza using integrated metagenomic and metabolomic analysis.
Findings
Indole-3-propionic acid (IPA) levels decrease during influenza infection and correlate with reduced Lactobacillaceae and Bifidobacteriaceae species.
Supplementing IPA in infected mice lowers viral load and inflammation, while targeting IPA-producing bacteria worsens outcomes.
IPA is negatively correlated with viral load and inflammation markers, suggesting a protective role in influenza.
Abstract
The gut-to-lung axis is critical during respiratory infections, including influenza A virus (IAV) infection. In the present study, we used high-resolution shotgun metagenomics and targeted metabolomic analysis to characterize influenza-associated changes in the composition and metabolism of the mouse gut microbiota. We observed several taxonomic-level changes on day (D)7 post-infection, including a marked reduction in the abundance of members of the Lactobacillaceae and Bifidobacteriaceae families, and an increase in the abundance of Akkermansia muciniphila. On D14, perturbation persisted in some species. Functional scale analysis of metagenomic data revealed transient changes in several metabolic pathways, particularly those leading to the production of short-chain fatty acids (SCFAs), polyamines, and tryptophan metabolites. Quantitative targeted metabolomics analysis of the serum…
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Taxonomy
TopicsNuclear Materials and Properties · Radiation Shielding Materials Analysis · Thermal and Kinetic Analysis