# Resistance to Frankliniella occidentalis during Different Plant Life Stages and under Different Environmental Conditions in the Ornamental Gladiolus

## TL;DR

This study examines how Gladiolus plants resist Western flower thrips at different growth stages and under various environmental conditions.

## Contribution

The study identifies consistent chemical resistance markers in Gladiolus across developmental stages and growing conditions.

## Key findings

- Robinetta variety showed significantly lower thrips damage compared to Charming Beauty across all developmental stages.
- Higher concentrations of triterpenoid saponins and amino acids in resistant Robinetta plants correlate with thrips resistance.
- Metabolomic profiles of leaves remained stable during plant development, while buds and flowers had higher amino acid concentrations.

## Abstract

The defense mechanisms of plants evolve as they develop. Previous research has identified chemical defenses against Western flower thrips (WFT) in Gladiolus (Gladiolus hybridus L.). Consequently, our study aimed to explore the consistency of these defense variations against WFT across the various developmental stages of Gladiolus grown under different conditions. Thrips bioassays were conducted on whole plants at three developmental stages, using the Charming Beauty and Robinetta varieties as examples of susceptible and resistant varieties, respectively. Metabolomic profiles of the leaves, buds and flowers before thrips infestation were analyzed. The thrips damage in Charming Beauty was more than 500-fold higher than the damage in Robinetta at all plant development stages. Relative concentrations of triterpenoid saponins and amino acids that were associated with resistance were higher in Robinetta at all plant stages. In Charming Beauty, the leaves exhibited greater damage compared to buds and flowers. The relative concentrations of alanine, valine and threonine were higher in buds and flowers than in leaves. The Metabolomic profiles of the leaves did not change significantly during plant development. In addition, we cultivated plants under different environmental conditions, ensuring consistency in the performance of the two varieties across different growing conditions. In conclusion, the chemical thrips resistance markers, based on the analysis of vegetative plants grown in climate rooms, were consistent over the plant’s lifetime and for plants grown under field conditions.