# Gene expression profiles of skin from cyclin dependent kinases 5-knockdown mice

## TL;DR

This study identifies genes regulated by CDK5 in mouse skin, revealing its role in hair pigmentation through TYRP1 and calcium signaling.

## Contribution

The study provides novel insights into CDK5's regulation of pigmentation-related genes and pathways in mouse skin.

## Key findings

- 8,002 known genes were differentially expressed in CDK5-knockdown mice compared to wild-type mice.
- TYRP1 and calcium signaling pathway are regulated by CDK5, linking it to hair pigmentation.
- 80 genes associated with hair color showed altered expression in CDK5-knockdown mice.

## Abstract

This study aimed to identify genes regulated by cyclin dependent kinases 5 (CDK5) that participate in hair pigmentation in mice.

The mRNA expression profiles of skin samples from CDK5-knockdown mice were constructed using high-throughput RNA sequencing and compared with those of wild-type mice.

In total, 8,002 known genes were differentially expressed between CDK5-knockdown and wild-type mice. Of these, 3,658 were upregulated and 4,344 were downregulated in the skin of CDK5-knockdown mice. An additional 318 previously unknown genes were also differentially expressed, with 171 downregulated and 147 upregulated genes in the skin of CDK5-knockdown mice. Of the known genes expressed in mouse skin, 80 were associated with hair color, with 61 showing lower expression and 19 exhibiting higher expression in skin of CDK5-knockdown mice. Importantly, the expression of the tyrosinase-related protein 1 (TYRP1) and the calcium signaling pathway were also found to be regulated by CDK5, suggesting that pigmentation is regulated by CDK5 via the calcium signaling pathway and TYRP1.

The transcriptome profiles obtained from the skin of CDK5-knockdown mice compared to wild-type mice provide a valuable resource to help understand the mechanism by which CDK5 regulates melanogenesis in mice and other animals.