# Maturation and development of fetal pig intestinal tissue in immunodeficient mice

## TL;DR

This study compares the development of fetal pig intestinal tissue and spheroids in mice, finding that segmental grafts mature better than spheroids.

## Contribution

The study demonstrates that fetal pig intestinal segmental grafts mature structurally in mice better than in-vitro spheroids.

## Key findings

- Spheroids lacked layered intestinal structure and showed little growth.
- Segmental grafts developed deep crypts and matured smooth muscle and nerve layers over time.
- Segmental grafts are more promising for transplantation than spheroids.

## Abstract

This study aimed to compare the degree of maturation and development of fetal pig segmental intestinal tissue with that of spheroids created by in-vitro reaggregation of dissociated fetal intestinal cells after transplantation into immunodeficient mice.

Fetal pig small intestines were transplanted as segmental grafts into the omentum and subrenal capsules of immunodeficient mice or enzymatically treated to generate single cells. Spheroids made by in-vitro reaggregation of these cells were transplanted into the subrenal capsules of immunodeficient mice. The segmental grafts and spheroids were harvested four and eight weeks after transplantation, and the structural maturity and in-vivo development of these specimens were histologically evaluated.

The spheroids were engrafted and supplied blood vessels from the host mice, but an intestinal layered structure was not clearly observed, and there was almost no change in size. On the other hand, the segmental grafts formed deep crypts in the mucus membrane, the inner circular layer, and outer longitudinal muscles. The crypts of the transplanted grafts harvested at eight weeks were much deeper, and the smooth muscle layer and the enteric nervous system were more mature than those of grafts harvested at the fourth week, although the intestinal peristaltic wave was not observed.

Spheroids created from fetal small intestinal cells could not form layered structures or mature sufficiently. Conversely, segmental tissues structurally matured and developed after in-vivo transplantation and are therefore potential grafts for transplantation.