Quality control on oil palm RNA samples for efficient genomic downstream applications
Ardha Apryianto, Primadiyanti Arsela, Foncha Felix, Walter Ajambang
TL;DR
This paper shows that RNA samples with poor initial quality measurements can still be used effectively in genomic studies after further quality checks.
Contribution
The study demonstrates that RNA samples should not be discarded based solely on preliminary field lab quality control.
Findings
RNA samples with poor OD values still produced excellent downstream genomic results.
RNA samples did not deteriorate during handling and manipulation stages.
Preliminary QC in field labs is insufficient to determine RNA sample usability.
Abstract
Quality control (QC) is primordial for determining the efficiency in any downstream genomic applications. There are several steps in the verification of the quality of RNA samples destined for genomic studies. The aim of this research was to determine whether RNA should be discarded at the level of the field lab if it fails preliminary quality control using Optical Density (OD) measurements. In this study, all samples were submitted to rigorous quality control in every stage of work. RNA samples showing poor OD values still gave excellent results in downstream QC and genomic applications. At the end of the quality control exercise, it was observed that the original samples were the same and had not undergone any deterioration along the different stages of handling and manipulation. This paper shows the different and most important stages of quality control on RNA samples (RIN) for an…
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Taxonomy
TopicsMolecular Biology Techniques and Applications · Genomics and Phylogenetic Studies · Coconut Research and Applications