# Thinking process templates for constructing data stories with SCDNEY

## TL;DR

This paper introduces workshops using scdney to guide single-cell data analysis through structured thinking templates and community collaboration.

## Contribution

The novel contribution is the development of living workshops and a thinking process template for structured single-cell data analysis.

## Key findings

- The first workshop focuses on cell phenotyping to understand cell identity and relationships.
- The second workshop extracts higher-order features to predict disease progression.
- The workshops use a collaborative, community-driven approach for dynamic learning.

## Abstract

Background: Globally, scientists now have the ability to generate a vast amount of high throughput biomedical data that carry critical information for important clinical and public health applications. This data revolution in biology is now creating a plethora of new single-cell datasets. Concurrently, there have been significant methodological advances in single-cell research. Integrating these two resources, creating tailor-made, efficient, and purpose-specific data analysis approaches can assist in accelerating scientific discovery.

Methods: We developed a series of living workshops for building data stories, using Single-cell data integrative analysis (scdney). scdney is a wrapper package with a collection of single-cell analysis R packages incorporating data integration, cell type annotation, higher order testing and more.

Results: Here, we illustrate two specific workshops. The first workshop examines how to characterise the identity and/or state of cells and the relationship between them, known as phenotyping. The second workshop focuses on extracting higher-order features from cells to predict disease progression.

Conclusions: Through these workshops, we not only showcase current solutions, but also highlight critical thinking points. In particular, we highlight the Thinking Process Template that provides a structured framework for the decision-making process behind such single-cell analyses. Furthermore, our workshop will incorporate dynamic contributions from the community in a collaborative learning approach, thus the term ‘living’.