# mSphere of Influence: The discovery of a missing link in bacterial cell envelope biogenesis

## TL;DR

This paper discusses how genetic studies revealed a new role for AsmA-family proteins in transporting lipids in bacteria, solving a longstanding question in bacterial physiology.

## Contribution

The discovery of AsmA-family proteins as key lipid transporters in Gram-negative bacteria fills a critical gap in understanding bacterial cell envelope biogenesis.

## Key findings

- AsmA-family proteins transport phospholipids between inner and outer membranes in Gram-negative bacteria.
- The studies used bacterial genetics to identify these proteins, answering a long-standing question in bacterial physiology.

## Abstract

Gregory Harrison is a bacteriologist researching essential pathways in bacteria as potential therapeutic targets. In this mSphere of Influence article, he reflects on a series of studies that employ complementary genetic approaches to define the crucial role of AsmA-family proteins in transporting phospholipids between the inner and outer membranes of Gram-negative bacteria. The authors of these three studies identify this family of lipid transporters through the means of bacterial genetics, answering a long-standing question in bacterial physiology, and serving as a reminder that a well-designed genetic strategy can go a long way in uncovering new biology.