# Bacillus anthracis, “la maladie du charbon”, Toxins, and Institut Pasteur

## TL;DR

This paper reviews 120 years of research on Bacillus anthracis at Institut Pasteur, highlighting its historical and scientific evolution.

## Contribution

The paper provides a historical analysis of B. anthracis research at Institut Pasteur, emphasizing its scientific and societal evolution.

## Key findings

- Early research focused on veterinary anthrax vaccines with limited understanding of molecular biology.
- Modern research adopted a multi-disciplinary approach, uncovering many aspects of B. anthracis pathogenicity and toxin mechanisms.
- Regulatory changes in the 21st century significantly impacted B. anthracis research practices.

## Abstract

Institut Pasteur and Bacillus anthracis have enjoyed a relationship lasting almost 120 years, starting from its foundation and the pioneering work of Louis Pasteur in the nascent fields of microbiology and vaccination, and blooming after 1986 following the molecular biology/genetic revolution. This contribution will give a historical overview of these two research eras, taking advantage of the archives conserved at Institut Pasteur. The first era mainly focused on the production, characterisation, surveillance and improvement of veterinary anthrax vaccines; the concepts and technologies with which to reach a deep understanding of this research field were not yet available. The second period saw a new era of B. anthracis research at Institut Pasteur, with the anthrax laboratory developing a multi-disciplinary approach, ranging from structural analysis, biochemistry, genetic expression, and regulation to bacterial-host cell interactions, in vivo pathogenicity, and therapy development; this led to the comprehensive unravelling of many facets of this toxi-infection. B. anthracis may exemplify some general points on how science is performed in a given society at a given time and how a scientific research domain evolves. A striking illustration can be seen in the additive layers of regulations that were implemented from the beginning of the 21st century and their impact on B. anthracis research. B. anthracis and anthrax are complex systems that raise many valuable questions regarding basic research. One may hope that B. anthracis research will be re-initiated under favourable circumstances later at Institut Pasteur.