# The Resistance of Soybean Variety Heinong 84 to Apple Latent Spherical Virus Is Controlled by Two Genetic Loci

## TL;DR

Researchers found that a soybean variety called Heinong 84 resists a virus due to two genetic regions, which could improve the use of this virus in genetic studies.

## Contribution

The study identifies two genetic loci controlling resistance to ALSV in soybean variety Heinong 84.

## Key findings

- Resistance to ALSV in Heinong 84 is controlled by two genetic loci on chromosomes 2 and 11.
- The resistance locus on chromosome 2 has a dominant dose-dependent role in inhibiting viral proliferation.
- A secondary resistance locus on chromosome 11 may encode a host factor required for viral proliferation.

## Abstract

Apple latent spherical virus (ALSV) is widely used as a virus-induced gene silencing (VIGS) vector for function genome study. However, the application of ALSV to soybeans is limited by the resistance of many varieties. In this study, the genetic locus linked to the resistance of a resistant soybean variety Heinong 84 was mapped by high-throughput sequencing-based bulk segregation analysis (HTS–BSA) using a hybrid population crossed from Heinong 84 and a susceptible variety, Zhonghuang 13. The results showed that the resistance of Heinong 84 to ALSV is controlled by two genetic loci located on chromosomes 2 and 11, respectively. Cleaved amplified polymorphic sequence (CAPS) markers were developed for identification and genotyping. Inheritance and biochemical analyses suggest that the resistance locus on chromosome 2 plays a dominant dose-dependent role, while the other locus contributes a secondary role in resisting ALSV. The resistance locus on chromosome 2 might encode a protein that can directly inhibit viral proliferation, while the secondary resistance locus on chromosome 11 may encode a host factor required for viral proliferation. Together, these data reveal novel insights on the resistance mechanism of Heinong 84 to ALSV, which will benefit the application of ALSV as a VIGS vector.