# Complete genome sequences of Mycobacterium smegmatis phages Ashballer and Bombitas **Authors:** Kristi M. Westover, Alexis R. Atkinson, Abby G. Bowers, Amaya L. Brown, James C. Ferebee, Chase A. Keisler, Kaylyn L. Lee, Amaya O. Payton, Lidia A. Peralta, Julianne V. Phu, Khamryn A. Pollock, Maya G. Scott, Bryson E. Vaughan, Karissa M. Wilczak, Victoria J. Frost PMC · DOI: 10.1128/mra.00990-23 · 2024-01-17 ## TL;DR This paper describes two new mycobacteriophages, Ashballer and Bombitas, isolated from soil with complete genome sequences. ## Contribution The novel contribution is the discovery and genome sequencing of two new temperate mycobacteriophages. ## Key findings - Ashballer has a genome length of 52,231 bp and siphovirus morphology. - Bombitas has a larger genome of 110,129 bp and also exhibits siphovirus morphology. - Both phages have temperate lifecycles. ## Abstract We report the discovery of two mycobacteriophages isolated from soil in Rock Hill, South Carolina. Ashballer has a genome sequence length of 52,231 bp, while Bombitas is relatively larger at 110,129 bp. Both have siphovirus morphologies and have temperate lifecycles. ## Full-text entities - **Species:** Mycolicibacterium smegmatis (species) [taxon 1772] ## Figures 1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC10868224/full.md --- Source: https://tomesphere.com/paper/PMC10868224