# From compost to culver: genome sequence and annotation of a cluster CQ1 Gordonia phage

**Authors:** William K. Alexander, Rianna R. Allen, Jaden D. Anderson, Amber N. Brumfield, Tiffany M. Cook, Giana M. Dana, Gregory J. Ethridge, Emily C. Gailey, Rebekah A. Netzley, Joshua V. Nguyen, Phillip J. Souza, Briggs M. Yoder, Jamie R. Wallen, Maria D. Gainey, Tonya C. Bates, Ellen M. Wisner

PMC · DOI: 10.1128/mra.01066-23 · 2023-12-22

## TL;DR

This paper presents the genome sequence and annotation of a new phage called Culver, isolated from Gordonia bacteria and belonging to the CQ1 cluster.

## Contribution

The study identifies unique genomic features of Culver, including two putative integrase genes not seen in other CQ1 phages.

## Key findings

- Culver is a siphovirus isolated from Gordonia terrae CAG3 and belongs to the CQ1 phage cluster.
- The genome encodes eight tRNAs and lysin A via two adjacent genes.
- Culver uniquely contains two putative integrase genes, distinguishing it from other CQ1 phages.

## Abstract

Phage Culver, with a siphovirus morphology, was isolated using Gordonia terrae CAG3. Culver is assigned to phage cluster CQ1 based on gene content similarity to actinobacteriophages. Notably, Culver is predicted to encode eight tRNAs, lysin A by two adjacent genes, and, unlike other CQ1 phages, two putative integrase genes.

## Linked entities

- **Genes:** trnaS (tRNA-Ser) [NCBI Gene 18667341]

## Full-text entities

- **Chemicals:** PYCa (-), copper (MESH:D003300), formvar (MESH:C013215), calcium (MESH:D002118), carbon (MESH:D002244), agar (MESH:D000362), uranyl acetate (MESH:C005460)
- **Species:** Saccharomyces cerevisiae (baker's yeast, species) [taxon 4932], Gordonia terrae (species) [taxon 2055], Gallus gallus (bantam, species) [taxon 9031]

## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC10868165/full.md

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Source: https://tomesphere.com/paper/PMC10868165