# Expansion of the split hygromycin toolkit for transgene insertion in Caenorhabditis elegans

## TL;DR

This paper expands a genetic tool for inserting transgenes in C. elegans, adding new chromosomal locations and natural isolates for better germline expression.

## Contribution

The split hygromycin system is extended to new chromosomal sites and natural isolates, enabling more flexible transgene insertion.

## Key findings

- Two new chromosomal locations permissive for germline expression were added to the split hygromycin system.
- Engineered landing pads were created in three additional natural isolates of C. elegans.
- Guide and repair template plasmids are now available for all expanded sites.

## Abstract

Engineered sites for genetic transformation have simplified transgene insertion in
Caenorhabditis elegans
. These strategies include our split hygromycin system ​(Stevenson et al. 2020)​ which allows for integration-specific selection of transgenes. Here we have expanded the split hygromycin selection system to include two additional chromosomal locations, both of which are permissive for germline expression, as well as engineered landing pads in three additional natural isolates. Corresponding guide and empty repair template plasmids are also available for each of these sites.