Melatonin Administration Enhances Testicular Volume, Testicular Blood Flow, Semen Parameters and Antioxidant Status during the Non-Breeding Season in Bafra Rams

TL;DR

Melatonin implants in rams during the non-breeding season improved testicular size, blood flow, and antioxidant levels, suggesting potential for boosting reproductive health.

Contribution

This study demonstrates that melatonin administration enhances testicular function in rams during the non-breeding season.

Findings

Melatonin increased testicular volume and blood flow in Bafra rams.

Melatonin reduced resistance and pulsatility indexes in testicular arteries.

Melatonin improved the total antioxidant status in treated rams.

Abstract

Our study aimed to understand how melatonin affects reproductive parameters in Bafra rams during the non-breeding season. One group of rams received melatonin implants, while another served as the control. We measured testicular volume, blood flow, and semen quality over several weeks. The results showed that melatonin increased testicular size and blood flow. This suggests that providing melatonin implants before the non-breeding season may improve reproductive capacity.

Our objectives were to investigate the effects of exogenous melatonin on testicular volume (TV), testicular blood flow (TBF), and semen quality in Bafra rams during the non-breeding season. One group of rams (MEL, n = 5) received a 36 mg melatonin implant twice, with 30 days in between, while the other group (CON, n = 5) served as the control. TBF, TV, and semen quality parameters were determined at three-week intervals starting three weeks before until twelve weeks after the first melatonin implant. Testicular blood flow was determined in the supratesticular (STA) and marginal testicular artery (MA) using color Doppler ultrasound. Semen was collected and evaluated, and the total oxidative status (TOS) and total antioxidative status (TAS) was determined using an ELISA. The MEL group had increased (p < 0.05) TV between the sixth and twelfth week after the start of treatment. Overall, the MEL group had lower resistance and pulsatility indexes (p < 0.05) between the third and ninth week, although there was no difference (p > 0.05) between the two groups in most semen quality parameters. However, TAS concentrations increased (p < 0.05) in the MEL group compared with the CON. The results of this study show that exogenous melatonin in the non-breeding season significantly increased both TBF and TV in Bafra rams. Therefore, giving rams implants with 36 mg melatonin twice at least one month prior to the non-breeding season is expected to improve testicular size and function and reproductive capacity.

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