# Caenorhabditis elegans ddx-15 helicase fails to complement loss of Prp43p in Saccharomyces cerevisiae

## TL;DR

This study shows that a helicase protein from C. elegans cannot replace a similar protein in yeast, suggesting functional differences despite conservation.

## Contribution

The novel finding is that DDX-15 from C. elegans fails to functionally complement Prp43p in yeast.

## Key findings

- DDX-15 from C. elegans cannot rescue the loss of Prp43p in yeast.
- Transcriptional repression experiments confirmed the functional incompatibility between DDX-15 and Prp43p.

## Abstract

Helicase proteins have important roles in many aspects of RNA metabolism in the cell. The function of these highly conserved proteins is commonly preserved between organisms, yet in a few cases these homologues are found to have slightly different biochemical functions. Prp43 is a protein with varied roles in yeast, but here we show that the
C. elegans
homologue of this protein is unable to rescue the loss of Prp43p. By employing a transcriptional repression experiment, the expression of DDX-15 protein in yeast is not enough to complement the loss of Prp43p, which is a yeast essential protein.