# Data on mitochondrial respiratory function in skeletal muscle of adult male mice in response to 3-weeks heat stress

## TL;DR

This study examines how 3 weeks of heat stress affects mitochondrial function in the skeletal muscle of adult male mice.

## Contribution

The study provides new data on mitochondrial respiratory function in skeletal muscle under chronic heat stress.

## Key findings

- Heat stress alters mitochondrial respiratory function in skeletal muscle.
- Heat stress impacts feeding, body weight, muscle weight, and heat shock protein expression.

## Abstract

Chronic heat stress induces mitochondrial adaptation in skeletal muscle. However, the effect of chronic heat stress on the respiratory function per mitochondria in skeletal muscle has not been well studied. Here, the present study reports on the effect of 3-weeks heat stress on muscle mitochondrial respiration using male C57BL/6JJ mice at age 21 weeks. Mice were randomly assigned to either the control group (n = 6) or passive heat group (n = 6). After 3-weeks of heat stress, the right triceps surae was removed and used for biochemical analysis. Protein expression was assessed by immunoblotting. Mitochondrial respiratory function was measured by Oxygraph-2k. The study also shows the impact of the heat stress on daily feeding, body weight, muscle weight, and protein expression of heat shock proteins (heat-response marker).