# Exploring TRF2-Dependent DNA Distortion Through Single-DNA Manipulation Studies

## TL;DR

This study reveals how TRF2 interacts with telomeric DNA, showing it can switch between different shapes and bend DNA under tension.

## Contribution

The study identifies TRF2's multiple DNA-binding modes and its role in DNA bending under tension.

## Key findings

- TRF2-DNA complexes can switch between extended and compact conformations.
- TRF2 does not strongly prefer DNA supercoiling chirality under low tension.
- TRF2 induces DNA bending when tension is applied.

## Abstract

TRF2 is a component of shelterin, a telomere-specific protein complex that protects the ends of mammalian chromosomes from DNA damage signaling and improper repair. TRF2 functions as a homodimer and its interaction with telomeric DNA has been studied, but its full-length DNA-binding properties are unknown. This study examines TRF2’s interaction with single-DNA strands and focuses on the conformation of the TRF2-DNA complex and TRF2’s preference for DNA chirality. The results show that TRF2-DNA can switch between extended and compact conformations, indicating multiple DNA-binding modes, and TRF2’s binding does not have a strong preference for DNA supercoiling chirality when DNA is under low tension. Instead, TRF2 induces DNA bending under tension. Furthermore, both the N-terminal domain of TRF2 and the Myb domain enhance its affinity for the telomere sequence, highlighting the crucial role of multivalent DNA binding in enhancing its affinity and specificity for telomere sequence. These discoveries offer unique insights into TRF2’s interaction with telomeric DNA.

Single-molecule DNA manipulations experiments reveal that the TRF2 component of the telomeric-specific complex exhibits multiple DNA-binding modes on telomeric DNA that can switch between extended and compact conformations with no strong preference for DNA supercoiling chirality under tension.