# Remote homology identification of the Drosophila melanogaster ortholog of the RNA Polymerase I subunit Rpa34/POLR1G

## TL;DR

This paper identifies a missing RNA polymerase subunit in fruit flies using structural and evolutionary analysis.

## Contribution

CG11076 is identified as the Drosophila ortholog of Rpa34/POLR1G using remote homology and structural analysis.

## Key findings

- CG11076 shows high structural conservation with Rpa34/POLR1G.
- Phylogenetic analysis confirms CG11076 is closely related to Rpa34/POLR1G.
- Combining sequence and structure improves identification of divergent orthologs.

## Abstract

Highly conserved orthologous proteins are easily identified by sequence homology alone, whereas poorly conserved orthologs require additional structural information to be identified. All
Drosophila
orthologs of RNA polymerase I, II, and III subunits—except one—have been identified by sequence homology. Here, we identified CG11076 as the missing Rpa34/POLR1G ortholog in
Drosophila
. Remote homology detection and secondary structure analysis showed that CG11076 is predicted to have high structural conservation with Rpa34/POLR1G, and phylogenetic analysis demonstrated that these proteins are closely related. Our work underscores the importance of utilizing both sequence and structure to identify highly divergent orthologous proteins in different species.