# The CRISPR/Cas9 system forms a condensate in the yeast nucleus

## TL;DR

The study shows that the CRISPR/Cas9 system forms a condensate in the nucleus of yeast cells, which could help improve gene editing techniques.

## Contribution

The novel contribution is the observation that Cas9 forms a condensate in the yeast nucleus, which co-localizes with the nucleolus.

## Key findings

- Cas9 accumulates in the nucleus of yeast cells over time.
- Cas9 forms a non-uniform condensate in the nucleoplasm.
- The condensate co-localizes with the nucleolus and targets the site to its periphery.

## Abstract

CRISPR/Cas9 gene editing technology has revolutionized genetic engineering. However, the nuclear dynamics of Cas9 in eukaryotic cells, particularly in the model organism
Saccharomyces cerevisiae
, remains poorly understood. Here, we constructed yeast strains expressing fluorescently tagged Cas9 variants, revealing their accumulation in the nucleus over time. Notably, Cas9 was non-uniformly distributed in the nucleoplasm during the initial hours, suggesting the formation of a condensate. This condensate often co-localizes with the nucleolus and associates the target site to its periphery. Our findings provide insights into Cas9 nuclear dynamics in yeast, advancing our understanding of CRISPR/Cas9-based genetic manipulation.