Intrinsic Brain Networks Underlying the Experience and Expression of Subclinical Anxiety

TL;DR

This study investigates how different components of subclinical anxiety are supported by distinct intrinsic brain networks using resting-state functional connectivity analysis.

Contribution

It identifies specific brain connectivity patterns associated with behavioral, physiological, and subjective anxiety components in subclinical individuals.

Findings

Higher subclinical anxiety linked to faster responses under threat.

ACC-insula connectivity related to behavioral responses.

ACC-OFC connectivity associated with physiological arousal.

Abstract

Anxiety includes behavioural, physiological, and subjective components that do not always align, and it remains unclear whether these dimensions are supported by distinct intrinsic brain networks. Guided by the two-system framework, we tested whether resting-state functional connectivity (rsFC) differentiates these components in subclinical anxiety. Forty-seven young adults spanning a range of subclinical anxiety levels completed a threat anticipation task measuring behavioral responses (reaction time) and physiological arousal (skin conductance), along with the NIH Fear-Affect self-report of anxiety severity. These measures were related to rsFC using region-of-interest analyses. Higher subclinical anxiety was associated with faster responses under temporally uncertain threat, consistent with increased vigilance, while no association was found with physiological arousal. At the neural level, three connectivity patterns emerged and remained significant after sequential family-wise error correction. Behavioural responses modulated by subclinical anxiety were linked to stronger connectivity between the anterior cingulate cortex (ACC) and insula. Physiological modulation was associated with connectivity between the ACC and orbitofrontal cortex (OFC). Subjective anxiety was associated with increased connectivity between the hippocampus and insula. Additional connections were observed but did not survive stricter correction. Overall, the findings indicate that behavioural, physiological, and subjective aspects of subclinical anxiety map onto partially dissociable but overlapping intrinsic brain networks, extending prior task-based results to resting-state connectivity and informing future work on early neural markers of anxiety.