A modelling perspective on mosquito infectiousness: time-varying transmission competence in arbovirus vector

TL;DR

This study introduces a time-varying model of mosquito infectiousness, revealing that transmission capacity may decline over time, challenging the traditional assumption of lifelong infectiousness in vector competence models.

Contribution

The paper extends existing intra-vector viral dynamics models by incorporating reversible transmission states, improving fit to experimental data and providing a more realistic representation of mosquito infectiousness.

Findings

Models with temporary transmission interruption fit data better in 7 of 10 decline cases.

Allowing transmission interruption increases estimates of mosquitoes crossing all barriers.

Intermittent transmission durations can last several days, similar to permanent cessation models.

Abstract

Mosquito vector competence is usually represented as a process in which once virus is detected in saliva, mosquitoes are assumed to remain infectious for life, implying an irreversible transition to the transmitting state. However, some experiments report declines in the proportion of transmitting mosquitoes at late times post-exposure, suggesting transmission capacity may not be permanent. To investigate this hypothesis, we extended a previously developed stochastic intra-vector viral dynamics model by introducing transmission states allowing either permanent cessation or temporary interruption of transmission. We fitted three competing models to data from 52 vector competence conditions covering chikungunya, dengue, Zika, West Nile, and Rift Valley fever viruses, using Approximate Bayesian Computation with Sequential Monte Carlo inference. Among the 10 experimental conditions showing decline in transmitter proportions, models allowing exit from the transmitting state provided a better fit in 7 cases, with clear improvement in 5. In these cases, allowing interruption of transmission increased posterior estimates of the proportion of mosquitoes that crossed all intra-mosquito barriers, whereas estimates of infected and disseminated state durations were largely unchanged. In cases where intermittent transmission was selected, its performance was similar to that of permanent cessation with non-transmitting periods lasting several days. These results indicate that the assumption of lifelong mosquito infectiousness does not always provide the best explanation for vector competence data and may lead to underestimation of the proportion of mosquitoes that become capable of transmission. Incorporating time-varying transmission competence into intra-vector models could improve interpretation of vector competence experiments and refine epidemiological representations of arbovirus transmission.