Engineering quantum optical responses of microtubules through tryptophan-network simulations and ultraviolet spectroscopy

TL;DR

This study combines simulations and experiments to explore how the optical responses of microtubules can be engineered by modifying their tryptophan networks, aiming to develop microtubule-based photonic applications.

Contribution

It introduces a combined theoretical and experimental approach to tune microtubule fluorescence through network modifications, providing design rules for engineered microtubule photonics.

Findings

Polymerization increases microtubule quantum yield at 280 nm.

Adding L-tryptophan quenches microtubule fluorescence.

Simulations show positional and orientational fluctuations affect radiative rates.

Abstract

Microtubules host dense ultraviolet-absorbing aromatic networks, suggesting an opportunity to engineer their optical response for biotechnology. Here we assess the feasibility of tuning microtubule fluorescence by combining an excitonic radiative-coupling model with molecular-dynamics-derived microtubule-like assemblies and steady-state absorbance and fluorescence measurements in microplate geometries. Simulations quantify how positional and orientational fluctuations reshape radiative rates and quantum yield, and predict how perturbing the tryptophan network by removing a specific site, adding an extra tryptophan at candidate binding pockets, or using mixed modification fractions can modulate emission. Experiments on porcine tubulin dimers and taxol-stabilized microtubules support these trends: polymerization enhances microtubule quantum yield at 280 nm and yields bounded changes at 295 nm due to scattering, while added L-tryptophan reproducibly quenches microtubules at both wavelengths. Together, theory and experiment provide evidence for chemically addressable tuning of microtubule quantum yield and motivate design rules for engineered microtubule photonics.