Nanodiamond sensing in dynamic environments with fast-tracking through four-point positioning

TL;DR

This paper introduces a fast four-point fluorescence tracking method for nanodiamonds with NV centers, enabling real-time, multi-parameter sensing in dynamic biological and fluidic environments.

Contribution

It presents a tetrahedral detection geometry with four APDs for rapid single-particle tracking, significantly improving temporal resolution over previous methods.

Findings

Achieved about tenfold increase in temporal resolution for nanodiamond tracking.

Demonstrated stable temperature and rotation sensing at various diffusion rates.

Enabled simultaneous translation and rotation tracking in live cells.

Abstract

Nitrogen-vacancy (NV) centers in nanodiamonds are excellent nanoscale sensors for measuring parameters such as temperature, magnetic field, and viscosity in complex fluidic environments, including living cells. However, the rapid motion of nanodiamonds in such dynamic systems imposes a significant challenge for continuous, real-time tracking and sensing measurements. Here, we present a fast single particle tracking (SPT) method featuring a tetrahedral detection geometry for time-efficient parallel fluorescence collection using four avalanche photodiodes (4-APDs), which eliminates the temporal latency of traditional sequential scanning. We demonstrate an improvement of about an order of magnitude in the temporal resolution and the upper limit of measurable diffusion coefficient compared to previously reported nanodiamond tracking methods based on single APD. The SPT is integrated with multi-parameter quantum sensing based on optically detected magnetic resonance (ODMR) of NV centers. The sensitivities of ODMR-based temperature and 3D rotation sensing are evaluated at different diffusion coefficients, which shows no significant degradation within our measurement range. We apply the system for thermorheology measurements in glycerol/water mixtures under thermal ramps. Additionally, we perform simultaneous translation and rotation tracking in live cells, revealing correlated translational and rotational dynamics. This approach advances multi-parameter nanoscale sensing for soft matter and biological applications, paving the way for real-time nanoscale sensing in highly dynamic fluidic environments.